Update for influenza virus, and other respiratory pathogen

- Background

For the upcoming 2016-2017 influenza season, the US CDC has announced the suggested influenza virus subtypes for vaccination (http://www.cdc.gov/flu/about/season/flu-season-2016-2017.htm). Guidelines on the use of rapid influenza diagnostic tests (RIDTs) from both WHO and CDC remain the same – RIDT results need to be validated by culture or RT-PCR to avoid potential false negative and positive results1,2.

- High sensitivity, fast and reliable RT-RCR test

RT-PCR assay kit with CE-IVD mark ensures the quality and reliability of it. The assay kits used for respiratory pathogen detection in our laboratory are all with CE-IVD mark whereas the detection limits of all assay kits reach one copy of viral/bacterial genome per one microliter specimen (1 copy/ul).

The multiplex realtime technology for RT-PCR allows us to detect up to 21 respiratory pathogens in one reaction, and the result is available within 24 hours (from the specimen arrives at the lab).

- Test Characteristics

The robustness of RT-PCR technology allow it to accommodate many kinds of specimens including nasopharyngeal swab, dry throat swab and sputum. The swabs DO NOT need any preservatives or transport medium, recent study show that RT-PCR results from plain dry swabs remain the same up to three days when they are kept and transported under ambient3. As a result, please keep the specimen in 4°C fridge and send to our lab within 3 days.

Test code

Detected Pathogens



Influenza Combo 3 (Influenza A, Influenza B, RSV A/B), Qualitative PCR



Influenza Combo 2 (Influenza A, Influenza B), Qualitative PCR



Influenza Combo 3 (Influenza A, Inf A(H1N1), Influenza B), Qualitative PCR



Influenza A differentiation (H1N1, H3, H5, H7), Qualitative PCR,
add-on item for influenza A positive cases



Respiratory Bacterial infection combo 7
(Streptococus pneumoniae, Haemophilius influenzae, Moraxella catarrhalis, Staphylococcus aureus, Mycoplasma pneumoniae, Chlamydia pneumoniae,
Legionella spp.)



Respiratory infection combo 21
(Influenza A, Influenza B, Influenza A (H1N1), rhinovirus, coronavirus NL63,229E,OC43,HKU1, parainfluenza1,2,3,4, MPV, bocavirus, RSV A/B, adenovirus, entrovirus, parechovirus, Mycoplasma pneumoniae)



- Guidance for Clinicians on the Use of Rapid Influenza Diagnostic Tests, Centers for Disease Control and Prevention, the U.S. Department of Health and Human Services. (http://www.cdc.gov/flu/professionals/diagnosis/clinician_guidance_ridt.htm)

- WHO recommendations on the use of rapid testing for influenza diagnosis, World Health Organization (http://www.who.int/influenza/resources/documents/rapid_testing/en/)

- Druce J, Garcia K, Tran T, Papadakis G, Birch C. Evaluation of swabs, transport media, and specimen transport conditions for optimal detection of viruses by PCR. J Clin Microbiol. 2012 Mar;50(3):1064-5. doi: 10.1128/JCM.06551-11.